ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Effect of nitric oxide synthase inhibitors in acute lung injury due to blunt lung trauma in rats
Aslı Gül Akgül1, Deniz Şahin2, Uğur Temel1, Aykut Eliçora3, Meltem Dillioğlugil4, Hale Maral Kır4, Özgür Doğa Özsoy4, Kürşat Yıldız5, Cüneyt Özer6, Salih Topçu7
1Department of Thoracic Surgery, Health Sciences University, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkey
2Department of Physiology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
3Department of Thoracic Surgery, Karaelmas Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey
4Department of Biochemistry, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
5Department of Pathology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
6Experimental Research Unit, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
7Department of Thoracic Surgery, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
DOI : 10.5606/tgkdc.dergisi.2019.15936
Background: This study aims to investigate the effects of blunt lung trauma performed in experimental rat model on lung tissue and blood as well as proinflammatory cytokines, oxidant-antioxidant enzymes and histopathological parameters after Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine administration.

Methods: The study included 50 adult male Wistar albino rats (weighing 350 to 400 g). Rats were randomly allocated into four groups. Except in the control, moderate-level pulmonary contusion was created in all other groups. Intraperitoneal saline solution was performed in groups 1 and 2, 25 Ngamma-nitro-L-arginine methyl ester in group 3, and 20 N-iminoethyl-L-ornithine in group 4. Blood and lung tissues were studied biochemically and histopathologically.

Results: Best outcomes were recorded statistically significantly in groups with administration of Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine when malondialdehyde response, mucous and histopathological values were examined. Significant improvement was detected in superoxide dismutase values in the group with administration of competitive nitric oxide synthase inhibitor Ngamma-nitro-L-arginine methyl ester. Nitric oxide values were substantially decreased in N-iminoethyl-L-ornithine group, while no significance was detected.

Conclusion: Free oxygen radicals and lipid peroxidation played a role in pulmonary contusion after blunt lung trauma. According to biochemical and histopathological outcomes, effects of inflammation were decreased and protective effects were formed with administration of both Ngammanitro- L-arginine methyl ester and N-iminoethyl-L-ornithine.

Keywords : Acute lung injury; oxidative stress; trauma
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